A rapid and sensitive radiochemical assay for phosphatidic acid phosphohydrolase activity

A rapid and sensitive radiochemical assay for phosphatidic acid phosphohydrolase activity

A technique is described for the radiochemical assay of phosphatidic acid phosphohydrolase activity in rat brain. Radiochemically pure 32P-labeled phosphatidic acid of known specific radioactivity and structure, which was biosynthesized in vitro by the diacylglycerol kinase of E. coli, was used as t...

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Main Authors: T J Flynn, D S Deshmukh, R A Pieringer
Format: Article
Language:English
Published: Elsevier 1977-11-01
Series:Journal of Lipid Research
Subjects:
diacylglycerol kinase of Escherichia coli
rat
brain
Online Access:http://www.sciencedirect.com/science/article/pii/S0022227520415974
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spelling doaj-00808bad2fa0416d9293d6841fad55c72021-04-24T05:53:46ZengElsevierJournal of Lipid Research0022-22751977-11-01186777780A rapid and sensitive radiochemical assay for phosphatidic acid phosphohydrolase activityT J Flynn0D S Deshmukh1R A Pieringer2Department of Biochemistry, Temple University School of Medicine, Philadelphia, PA 19140; Department of Biology, Temple University, Philadelphia, PA 19122Department of Biochemistry, Temple University School of Medicine, Philadelphia, PA 19140; N.Y. Institute for Research in Mental Retardation, Staten Island, NY 10314Department of Biochemistry, Temple University School of Medicine, Philadelphia, PA 19140A technique is described for the radiochemical assay of phosphatidic acid phosphohydrolase activity in rat brain. Radiochemically pure 32P-labeled phosphatidic acid of known specific radioactivity and structure, which was biosynthesized in vitro by the diacylglycerol kinase of E. coli, was used as the substrate. As little as 5 µg of microsomal or mitochondrial protein can be used for the assay, and product formation in the picomole range can be determined accurately. This procedure should be useful in situations where only limited amounts of tissue are available.http://www.sciencedirect.com/science/article/pii/S0022227520415974diacylglycerol kinase of Escherichia coliratbrain
collection DOAJ
language English
format Article
sources DOAJ
author T J Flynn
D S Deshmukh
R A Pieringer
spellingShingle T J Flynn
D S Deshmukh
R A Pieringer
A rapid and sensitive radiochemical assay for phosphatidic acid phosphohydrolase activity
Journal of Lipid Research
diacylglycerol kinase of Escherichia coli
rat
brain
author_facet T J Flynn
D S Deshmukh
R A Pieringer
author_sort T J Flynn
title A rapid and sensitive radiochemical assay for phosphatidic acid phosphohydrolase activity
title_short A rapid and sensitive radiochemical assay for phosphatidic acid phosphohydrolase activity
title_full A rapid and sensitive radiochemical assay for phosphatidic acid phosphohydrolase activity
title_fullStr A rapid and sensitive radiochemical assay for phosphatidic acid phosphohydrolase activity
title_full_unstemmed A rapid and sensitive radiochemical assay for phosphatidic acid phosphohydrolase activity
title_sort rapid and sensitive radiochemical assay for phosphatidic acid phosphohydrolase activity
publisher Elsevier
series Journal of Lipid Research
issn 0022-2275
publishDate 1977-11-01
description A technique is described for the radiochemical assay of phosphatidic acid phosphohydrolase activity in rat brain. Radiochemically pure 32P-labeled phosphatidic acid of known specific radioactivity and structure, which was biosynthesized in vitro by the diacylglycerol kinase of E. coli, was used as the substrate. As little as 5 µg of microsomal or mitochondrial protein can be used for the assay, and product formation in the picomole range can be determined accurately. This procedure should be useful in situations where only limited amounts of tissue are available.
topic diacylglycerol kinase of Escherichia coli
rat
brain
url http://www.sciencedirect.com/science/article/pii/S0022227520415974
work_keys_str_mv AT tjflynn arapidandsensitiveradiochemicalassayforphosphatidicacidphosphohydrolaseactivity
AT dsdeshmukh arapidandsensitiveradiochemicalassayforphosphatidicacidphosphohydrolaseactivity
AT rapieringer arapidandsensitiveradiochemicalassayforphosphatidicacidphosphohydrolaseactivity
AT tjflynn rapidandsensitiveradiochemicalassayforphosphatidicacidphosphohydrolaseactivity
AT dsdeshmukh rapidandsensitiveradiochemicalassayforphosphatidicacidphosphohydrolaseactivity
AT rapieringer rapidandsensitiveradiochemicalassayforphosphatidicacidphosphohydrolaseactivity
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