Derivation of Huntington Disease affected Genea046 human embryonic stem cell line

Derivation of Huntington Disease affected Genea046 human embryonic stem cell line

The Genea046 human embryonic stem cell line was derived from a donated, fully commercially consented ART blastocyst, carrying HTT gene CAG expansion of 45 repeats, indicative of Huntington Disease. Following ICM outgrowth on inactivated human feeders, karyotype was confirmed as 46, XX by CGH and STR...

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Main Authors: Biljana Dumevska, Omar Chami, Robert McKernan, Divya Goel, Uli Schmidt
Format: Article
Language:English
Published: Elsevier 2016-03-01
Series:Stem Cell Research
Online Access:http://www.sciencedirect.com/science/article/pii/S1873506116000635
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spelling doaj-0079e72930ed40519712ab1b494729b32020-11-24T23:01:32ZengElsevierStem Cell Research1873-50611876-77532016-03-0116244644810.1016/j.scr.2016.02.021Derivation of Huntington Disease affected Genea046 human embryonic stem cell lineBiljana DumevskaOmar ChamiRobert McKernanDivya GoelUli SchmidtThe Genea046 human embryonic stem cell line was derived from a donated, fully commercially consented ART blastocyst, carrying HTT gene CAG expansion of 45 repeats, indicative of Huntington Disease. Following ICM outgrowth on inactivated human feeders, karyotype was confirmed as 46, XX by CGH and STR analysis demonstrated a female Allele pattern. The hESC line had pluripotent cell morphology, 85% of cells expressed Nanog, 92% Oct4, 75% Tra1–60 and 99% SSEA4 and demonstrated Alkaline Phosphatase activity. The cell line was negative for Mycoplasma and visible contamination.http://www.sciencedirect.com/science/article/pii/S1873506116000635
collection DOAJ
language English
format Article
sources DOAJ
author Biljana Dumevska
Omar Chami
Robert McKernan
Divya Goel
Uli Schmidt
spellingShingle Biljana Dumevska
Omar Chami
Robert McKernan
Divya Goel
Uli Schmidt
Derivation of Huntington Disease affected Genea046 human embryonic stem cell line
Stem Cell Research
author_facet Biljana Dumevska
Omar Chami
Robert McKernan
Divya Goel
Uli Schmidt
author_sort Biljana Dumevska
title Derivation of Huntington Disease affected Genea046 human embryonic stem cell line
title_short Derivation of Huntington Disease affected Genea046 human embryonic stem cell line
title_full Derivation of Huntington Disease affected Genea046 human embryonic stem cell line
title_fullStr Derivation of Huntington Disease affected Genea046 human embryonic stem cell line
title_full_unstemmed Derivation of Huntington Disease affected Genea046 human embryonic stem cell line
title_sort derivation of huntington disease affected genea046 human embryonic stem cell line
publisher Elsevier
series Stem Cell Research
issn 1873-5061
1876-7753
publishDate 2016-03-01
description The Genea046 human embryonic stem cell line was derived from a donated, fully commercially consented ART blastocyst, carrying HTT gene CAG expansion of 45 repeats, indicative of Huntington Disease. Following ICM outgrowth on inactivated human feeders, karyotype was confirmed as 46, XX by CGH and STR analysis demonstrated a female Allele pattern. The hESC line had pluripotent cell morphology, 85% of cells expressed Nanog, 92% Oct4, 75% Tra1–60 and 99% SSEA4 and demonstrated Alkaline Phosphatase activity. The cell line was negative for Mycoplasma and visible contamination.
url http://www.sciencedirect.com/science/article/pii/S1873506116000635
work_keys_str_mv AT biljanadumevska derivationofhuntingtondiseaseaffectedgenea046humanembryonicstemcellline
AT omarchami derivationofhuntingtondiseaseaffectedgenea046humanembryonicstemcellline
AT robertmckernan derivationofhuntingtondiseaseaffectedgenea046humanembryonicstemcellline
AT divyagoel derivationofhuntingtondiseaseaffectedgenea046humanembryonicstemcellline
AT ulischmidt derivationofhuntingtondiseaseaffectedgenea046humanembryonicstemcellline
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