Determination of Silver Ions Toxicity in Short-Term and Long-Term Experiments Using a Luminescent Recombinant Strain of E. coli

Determination of Silver Ions Toxicity in Short-Term and Long-Term Experiments Using a Luminescent Recombinant Strain of E. coli

The effects of silver ions on the luminescent recombinant strain of Escherichia coli carrying luxCDABE operon of Vibrio fischeri were investigated. The toxicity of silver ions was determined in 30 minutes and in chronic 24 hours experiments. Changes in the luminescence intensity and in the growth ra...

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Main Authors: Tatiana P. Yudina, Elena V. Sorokina, Mikhail M. Mazhul, Vadim S. Danilov
Format: Article
Language:Russian
Published: Academic Publishing House Researcher 2013-01-01
Series:Evropejskij Issledovatelʹ
Subjects:
silver ions
bacterial luminescence test
E. Coli
toxicity
bacterial growth
Online Access:http://www.erjournal.ru/pdf.html?n=1362429022.pdf
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spelling doaj-005dfb20dde74022abdb98316819d3a62020-11-24T23:32:23ZrusAcademic Publishing House ResearcherEvropejskij Issledovatelʹ2219-82292224-01362013-01-01422-3409417Determination of Silver Ions Toxicity in Short-Term and Long-Term Experiments Using a Luminescent Recombinant Strain of E. coliTatiana P. YudinaElena V. SorokinaMikhail M. MazhulVadim S. DanilovThe effects of silver ions on the luminescent recombinant strain of Escherichia coli carrying luxCDABE operon of Vibrio fischeri were investigated. The toxicity of silver ions was determined in 30 minutes and in chronic 24 hours experiments. Changes in the luminescence intensity and in the growth rate of bacteria were considered as a measure of silver ions toxicity within the range of concentrations applied. The effect of silver ions was demonstrated to be strongly dependent on the concentration of bacteria and on the medium composition. EC50 values were 0.018 mg/l after 30 min exposure and 0.014 mg/l after 10 hours of bacterial growth. Comparison of two modifications of the experiment showed that silver ions have a strong non-specific toxicity, as well as a specific effect on bacterial cellshttp://www.erjournal.ru/pdf.html?n=1362429022.pdfsilver ionsbacterial luminescence testE. Colitoxicitybacterial growth
collection DOAJ
language Russian
format Article
sources DOAJ
author Tatiana P. Yudina
Elena V. Sorokina
Mikhail M. Mazhul
Vadim S. Danilov
spellingShingle Tatiana P. Yudina
Elena V. Sorokina
Mikhail M. Mazhul
Vadim S. Danilov
Determination of Silver Ions Toxicity in Short-Term and Long-Term Experiments Using a Luminescent Recombinant Strain of E. coli
Evropejskij Issledovatelʹ
silver ions
bacterial luminescence test
E. Coli
toxicity
bacterial growth
author_facet Tatiana P. Yudina
Elena V. Sorokina
Mikhail M. Mazhul
Vadim S. Danilov
author_sort Tatiana P. Yudina
title Determination of Silver Ions Toxicity in Short-Term and Long-Term Experiments Using a Luminescent Recombinant Strain of E. coli
title_short Determination of Silver Ions Toxicity in Short-Term and Long-Term Experiments Using a Luminescent Recombinant Strain of E. coli
title_full Determination of Silver Ions Toxicity in Short-Term and Long-Term Experiments Using a Luminescent Recombinant Strain of E. coli
title_fullStr Determination of Silver Ions Toxicity in Short-Term and Long-Term Experiments Using a Luminescent Recombinant Strain of E. coli
title_full_unstemmed Determination of Silver Ions Toxicity in Short-Term and Long-Term Experiments Using a Luminescent Recombinant Strain of E. coli
title_sort determination of silver ions toxicity in short-term and long-term experiments using a luminescent recombinant strain of e. coli
publisher Academic Publishing House Researcher
series Evropejskij Issledovatelʹ
issn 2219-8229
2224-0136
publishDate 2013-01-01
description The effects of silver ions on the luminescent recombinant strain of Escherichia coli carrying luxCDABE operon of Vibrio fischeri were investigated. The toxicity of silver ions was determined in 30 minutes and in chronic 24 hours experiments. Changes in the luminescence intensity and in the growth rate of bacteria were considered as a measure of silver ions toxicity within the range of concentrations applied. The effect of silver ions was demonstrated to be strongly dependent on the concentration of bacteria and on the medium composition. EC50 values were 0.018 mg/l after 30 min exposure and 0.014 mg/l after 10 hours of bacterial growth. Comparison of two modifications of the experiment showed that silver ions have a strong non-specific toxicity, as well as a specific effect on bacterial cells
topic silver ions
bacterial luminescence test
E. Coli
toxicity
bacterial growth
url http://www.erjournal.ru/pdf.html?n=1362429022.pdf
work_keys_str_mv AT tatianapyudina determinationofsilverionstoxicityinshorttermandlongtermexperimentsusingaluminescentrecombinantstrainofecoli
AT elenavsorokina determinationofsilverionstoxicityinshorttermandlongtermexperimentsusingaluminescentrecombinantstrainofecoli
AT mikhailmmazhul determinationofsilverionstoxicityinshorttermandlongtermexperimentsusingaluminescentrecombinantstrainofecoli
AT vadimsdanilov determinationofsilverionstoxicityinshorttermandlongtermexperimentsusingaluminescentrecombinantstrainofecoli
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