Nitrogen Addition Changes the Stoichiometry and Growth Rate of Different Organs in Pinus tabuliformis Seedlings

• Main Authors: Hang Jing, Haoxiang Zhou, Guoliang Wang, Sha Xue, Guobin Liu, Mengcheng Duan
• Format: Article
• Language: English
• Published: Frontiers Media S.A. 2017-11-01
• Series: Frontiers in Plant Science
• Subjects: nitrogen addition; stoichiometry; growth rate; plant organ; root
• Online Access: http://journal.frontiersin.org/article/10.3389/fpls.2017.01922/full

Background: Nitrogen (N) deposition could influence plant stoichiometry and growth rate and thus alter the structure and function of the ecosystem. However, the mechanism by which N deposition changes the stoichiometry and relative growth rate (RGR) of plant organs, especially roots with different diameters, is unclear.Methods: We created a gradient of N availability (0–22.4 g N m-2 year-1) for Pinus tabuliformis seedlings for 3 years and examined changes in the carbon (C):N:phosphorus (P) ratios and RGRs of the leaves, stems, and roots with four diameter classes (finest roots, <0.5 mm; finer roots, 0.5–1 mm; middle roots, 1–2 mm; and coarse roots, >2 mm).Results: (1) N addition significantly increased the C and N contents of the leaves and whole roots, the C content of the stems, the N:P ratios of the leaves and stems, and the C:P ratio of the whole roots. (2) In the root system, the C:N ratio of the finest roots and the C:P ratios of the finest and finer roots significantly changed with N addition. The N:P ratios of the finest, finer, and middle roots significantly increased with increasing amount of N added. The stoichiometric responses of the roots were more sensitive to N addition than those of the other organs (3) The RGR of all the organs significantly increased at low N addition levels (2.8–11.2 g N m-2 year-1) but decreased at high N addition levels (22.4 g N m-2 year-1). (4) The RGRs of the whole seedlings and leaves were not significantly correlated with their N:P ratios at low and high N addition levels. By contrast, the RGRs of the stems and roots showed a significantly positive correlation with their own N:P ratio only at low N addition level.Conclusion: Addition of N affected plant growth by altering the contents of C and N; the ratios of C, N, and P; and the RGRs of the organs. RGR is correlated with the N:P ratios of the stems and roots at low N addition level but not at high N addition level. This finding is inconsistent with the growth rate hypothesis.