In vitro and in vivo enhanced osteogenesis by kaempferol found by a high-throughput assay using human mesenchymal stromal cells
High-throughput screening was performed as a phenotypic screening on clinically relevant human mesenchymal stem cells to identify small molecules that affect stem cell fate. From a library of pharmacologically active small molecules, we identified kaempferol, which is a natural flavonol, with a high...
| Main Authors: | , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2014-01-01
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| Series: | Journal of Functional Foods |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S1756464613002417 |
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doaj-0041d74b11cb4ee69bf2fd92f23ab1b6 |
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| record_format |
Article |
| collection |
DOAJ |
| language |
English |
| format |
Article |
| sources |
DOAJ |
| author |
Tetsuro Mazaki Takashi Kitajima Yasuyuki Shiozaki Miwa Sato Megumi Mino Aki Yoshida Mariko Nakamura Yasuhiro Yoshida Masato Tanaka Toshifumi Ozaki Akihiro Matsukawa Yoshihiro Ito |
| spellingShingle |
Tetsuro Mazaki Takashi Kitajima Yasuyuki Shiozaki Miwa Sato Megumi Mino Aki Yoshida Mariko Nakamura Yasuhiro Yoshida Masato Tanaka Toshifumi Ozaki Akihiro Matsukawa Yoshihiro Ito In vitro and in vivo enhanced osteogenesis by kaempferol found by a high-throughput assay using human mesenchymal stromal cells Journal of Functional Foods Kaempferol Ipriflavone Flavonoids High-throughput screening Mesenchymal stem cell Osteoporosis |
| author_facet |
Tetsuro Mazaki Takashi Kitajima Yasuyuki Shiozaki Miwa Sato Megumi Mino Aki Yoshida Mariko Nakamura Yasuhiro Yoshida Masato Tanaka Toshifumi Ozaki Akihiro Matsukawa Yoshihiro Ito |
| author_sort |
Tetsuro Mazaki |
| title |
In vitro and in vivo enhanced osteogenesis by kaempferol found by a high-throughput assay using human mesenchymal stromal cells |
| title_short |
In vitro and in vivo enhanced osteogenesis by kaempferol found by a high-throughput assay using human mesenchymal stromal cells |
| title_full |
In vitro and in vivo enhanced osteogenesis by kaempferol found by a high-throughput assay using human mesenchymal stromal cells |
| title_fullStr |
In vitro and in vivo enhanced osteogenesis by kaempferol found by a high-throughput assay using human mesenchymal stromal cells |
| title_full_unstemmed |
In vitro and in vivo enhanced osteogenesis by kaempferol found by a high-throughput assay using human mesenchymal stromal cells |
| title_sort |
in vitro and in vivo enhanced osteogenesis by kaempferol found by a high-throughput assay using human mesenchymal stromal cells |
| publisher |
Elsevier |
| series |
Journal of Functional Foods |
| issn |
1756-4646 |
| publishDate |
2014-01-01 |
| description |
High-throughput screening was performed as a phenotypic screening on clinically relevant human mesenchymal stem cells to identify small molecules that affect stem cell fate. From a library of pharmacologically active small molecules, we identified kaempferol, which is a natural flavonol, with a high osteogenic activity. Kaempferol achieved the highest levels of bone-specific markers among the chemicals tested in vitro. The osteogenic activity of kaempferol was the same as that of ipriflavone, which is a synthetic isoflavone, in ovariectomized mouse experiments. Considering that kaempferol is present in various foods such as vegetables and fruits, it will be useful as a new preventive medicine for osteoporosis. |
| topic |
Kaempferol Ipriflavone Flavonoids High-throughput screening Mesenchymal stem cell Osteoporosis |
| url |
http://www.sciencedirect.com/science/article/pii/S1756464613002417 |
| work_keys_str_mv |
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doaj-0041d74b11cb4ee69bf2fd92f23ab1b62021-04-29T04:41:46ZengElsevierJournal of Functional Foods1756-46462014-01-016241247In vitro and in vivo enhanced osteogenesis by kaempferol found by a high-throughput assay using human mesenchymal stromal cellsTetsuro Mazaki0Takashi Kitajima1Yasuyuki Shiozaki2Miwa Sato3Megumi Mino4Aki Yoshida5Mariko Nakamura6Yasuhiro Yoshida7Masato Tanaka8Toshifumi Ozaki9Akihiro Matsukawa10Yoshihiro Ito11Department of Orthopaedic Surgery, Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University, 2-5-1 Shikata-cho, Kita-ku, Okayama 700-8558, Japan; Department of Pathology and Experimental Medicine, Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University, 2-5-1 Shikata-cho, Kita-ku, Okayama 700-8558, JapanNano Medical Engineering Laboratory, RIKEN, 2-1 Hirosawa, Wako 351-0198, Japan; Molecular Ligand Biology Research Team, Chemical Genomics Research Group, Chemical Biology Department, RIKEN Advanced Science Institute, 2-1 Hirosawa, Wako 351-0198, JapanDepartment of Orthopaedic Surgery, Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University, 2-5-1 Shikata-cho, Kita-ku, Okayama 700-8558, Japan; Department of Pathology and Experimental Medicine, Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University, 2-5-1 Shikata-cho, Kita-ku, Okayama 700-8558, JapanDepartment of Pathology and Experimental Medicine, Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University, 2-5-1 Shikata-cho, Kita-ku, Okayama 700-8558, JapanDepartment of Pathology and Experimental Medicine, Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University, 2-5-1 Shikata-cho, Kita-ku, Okayama 700-8558, JapanDepartment of Orthopaedic Surgery, Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University, 2-5-1 Shikata-cho, Kita-ku, Okayama 700-8558, JapanDepartment of Pathology and Experimental Medicine, Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University, 2-5-1 Shikata-cho, Kita-ku, Okayama 700-8558, Japan; Department of Health and Welfare Program, Kibi International University Junior College, 8 Iga-machi, Takahashi, Okayama 716-8508, JapanDepartment of Biomaterials, Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University, 2-5-1 Shikata-cho, Kita-ku, Okayama 700-8558, JapanDepartment of Orthopaedic Surgery, Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University, 2-5-1 Shikata-cho, Kita-ku, Okayama 700-8558, JapanDepartment of Orthopaedic Surgery, Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University, 2-5-1 Shikata-cho, Kita-ku, Okayama 700-8558, JapanDepartment of Pathology and Experimental Medicine, Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University, 2-5-1 Shikata-cho, Kita-ku, Okayama 700-8558, JapanNano Medical Engineering Laboratory, RIKEN, 2-1 Hirosawa, Wako 351-0198, Japan; Molecular Ligand Biology Research Team, Chemical Genomics Research Group, Chemical Biology Department, RIKEN Advanced Science Institute, 2-1 Hirosawa, Wako 351-0198, Japan; Corresponding author at: Nano Medical Engineering Laboratory, RIKEN, 2-1 Hirosawa, Wako 351-0198, Japan. Tel.: +81 48 467 5809; fax: +81 48 467 9300.High-throughput screening was performed as a phenotypic screening on clinically relevant human mesenchymal stem cells to identify small molecules that affect stem cell fate. From a library of pharmacologically active small molecules, we identified kaempferol, which is a natural flavonol, with a high osteogenic activity. Kaempferol achieved the highest levels of bone-specific markers among the chemicals tested in vitro. The osteogenic activity of kaempferol was the same as that of ipriflavone, which is a synthetic isoflavone, in ovariectomized mouse experiments. Considering that kaempferol is present in various foods such as vegetables and fruits, it will be useful as a new preventive medicine for osteoporosis.http://www.sciencedirect.com/science/article/pii/S1756464613002417KaempferolIpriflavoneFlavonoidsHigh-throughput screeningMesenchymal stem cellOsteoporosis |