Cytoskeletal tension and Bazooka tune interface geometry to ensure fusion fidelity and sheet integrity during dorsal closure

• Main Authors: Piyal Taru Das Gupta, Maithreyi Narasimha
• Format: Article
• Language: English
• Published: eLife Sciences Publications Ltd 2019-04-01
• Series: eLife
• Subjects: reepithelialisation; precision; symmetry; embryonic segments; junction remodeling; emergent mechanisms
• Online Access: https://elifesciences.org/articles/41091

Epithelial fusion establishes continuity between the separated flanks of epithelial sheets. Despite its importance in creating resilient barriers, the mechanisms that ensure stable continuity and preserve morphological and molecular symmetry upon fusion remain unclear. Using the segmented embryonic epidermis whose flanks fuse during Drosophila dorsal closure, we demonstrate that epidermal flanks modulate cell numbers and geometry of their fusing fronts to achieve fusion fidelity. While fusing flanks become more matched for both parameters before fusion, differences persisting at fusion are corrected by modulating fusing front width within each segment to ensure alignment of segment boundaries. We show that fusing cell interfaces are remodelled from en-face contacts at fusion to an interlocking arrangement after fusion, and demonstrate that changes in interface length and geometry are dependent on the spatiotemporal regulation of cytoskeletal tension and Bazooka/Par3. Our work uncovers genetically constrained and mechanically triggered adaptive mechanisms contributing to fusion fidelity and epithelial continuity.