Treatment with surfactants enables quantification of translational activity by O-propargyl-puromycin labelling in yeast

by Jennifer Staudacher, Corinna Rebnegger, Brigitte Gasser
Published 2021-04-01

Disruption of vacuolar protein sorting components of the HOPS complex leads to enhanced secretion of recombinant proteins in Pichia pastoris

by Lukas Marsalek, Verena Puxbaum, Markus Buchetics, Diethard Mattanovich, Brigitte Gasser
Published 2019-07-01

Biomarkers allow detection of nutrient limitations and respective supplementation for elimination in Pichia pastoris fed-batch cultures

by Jonas Burgard, Minoska Valli, Alexandra B. Graf, Brigitte Gasser, Diethard Mattanovich
Published 2017-07-01

The secretome of Pichia pastoris in fed‐batch cultivations is largely independent of the carbon source but changes quantitatively over cultivation time

by Jonas Burgard, Clemens Grünwald‐Gruber, Friedrich Altmann, Jürgen Zanghellini, Minoska Valli, Diethard Mattanovich, Brigitte Gasser
Published 2020-03-01

Overexpression of the transcription factor Yap1 modifies intracellular redox conditions and enhances recombinant protein secretion

by Marizela Delic, Alexandra B. Graf, Gunda Koellensperger, Christina Haberhauer-Troyer, Stephan Hann, Diethard Mattanovich, Brigitte Gasser
Published 2014-10-01

A gene optimization strategy that enhances production of fully functional P-glycoprotein in Pichia pastoris.

by Jiangping Bai, Douglas J Swartz, Irina I Protasevich, Christie G Brouillette, Patina M Harrell, Ellen Hildebrandt, Brigitte Gasser, Diethard Mattanovich, Andrew Ward, Geoffrey Chang, Ina L Urbatsch
Published 2011-01-01